5T1J

Crystal Structure of the Tbox DNA binding domain of the transcription factor T-bet

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-08
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.98
Spacegroup name	P 61
Unit cell lengths	70.450, 70.450, 438.389
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.702 - 2.947
R-factor	0.2674
Rwork	0.266
R-free	0.29440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.741
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.990
High resolution limit [Å]	2.940	7.970	2.940
Rmerge	0.124	0.080	0.848
Number of reflections	22707
<I/σ(I)>	15.7
Completeness [%]	87.3	85.1	31.4
Redundancy	8.7	8.8	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.1	293	sodium citrate, sodium formate, ethylene glycol