5SZM
Protocadherin gamma A8 extracellular cadherin domains 1-4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 257.560, 257.560, 105.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.938 - 3.600 |
| R-factor | 0.2122 |
| Rwork | 0.211 |
| R-free | 0.23780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zps |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.564 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PH |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.510 | 3.970 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.172 | 0.913 |
| Number of reflections | 20729 | |
| <I/σ(I)> | 8.6 | 2.3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.1 | 7.3 |
| CC(1/2) | 0.998 | 0.864 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.5 | 295 | 11% isopropanol, 50 mM sodium chloride, 0.1 M Hepes pH 7.5 |
