5SZK

Structure of human N-terminally engineered Rab1b in complex with the bMERB domain of Mical-cL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91908
Spacegroup name	C 2 2 21
Unit cell lengths	62.230, 117.030, 139.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.800 - 2.800
R-factor	0.2102
Rwork	0.207
R-free	0.26070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nkv 5szg
RMSD bond length	0.010
RMSD bond angle	1.178
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10-2155_1692: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.800	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.076	1.110
Number of reflections	12904
<I/σ(I)>	22.8	2.45
Completeness [%]	100.0	100
Redundancy	19.2	13.8
CC(1/2)		0.757

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1M bis-tris pH 7.5, 0.2M sodium malonate and 20% (w/v) PEG3350