5SZI
Structure of human Rab8a in complex with the bMERB domain of Mical-cL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00009 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 62.400, 122.400, 139.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.952 - 2.850 |
| R-factor | 0.2396 |
| Rwork | 0.237 |
| R-free | 0.28840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lhw 5szg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.175 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10-2155_1692: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 2.950 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.089 | 0.780 |
| Number of reflections | 12810 | |
| <I/σ(I)> | 16.45 | 3.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12.7 | 13.5 |
| CC(1/2) | 0.507 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M bis tris propane 0.2M tri sodium citrate 20% PEG 3350 |
