5SXP
STRUCTURAL BASIS FOR THE INTERACTION BETWEEN ITCH PRR AND BETA-PIX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 28.845, 43.451, 61.482 |
| Unit cell angles | 90.37, 101.01, 105.25 |
Refinement procedure
| Resolution | 41.847 - 1.650 |
| R-factor | 0.1398 |
| Rwork | 0.139 |
| R-free | 0.16130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p4r |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.714 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.021 | 0.069 |
| Number of reflections | 32525 | |
| <I/σ(I)> | 18.2 | 6.9 |
| Completeness [%] | 95.7 | 93.2 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM MIB buffer pH 5.0 and 25% PEG1500 |
