5SXH

Crystal Structure of the Cancer Genomic DNA Mutator APOBEC3B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	44.390, 50.990, 77.410
Unit cell angles	90.00, 101.96, 90.00

Refinement procedure

Resolution	41.570 - 1.780
R-factor	0.1869
Rwork	0.185
R-free	0.21570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cqk
RMSD bond length	0.004
RMSD bond angle	0.762
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	phenix.refine (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.550	1.830
High resolution limit [Å]	1.780	1.780
Rmerge	0.102	1.100
Number of reflections	32228
<I/σ(I)>	8.8	1
Completeness [%]	98.9	99.1
Redundancy	3.8	3.8
CC(1/2)		0.376

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	PEG3350