5SWW

Crystal Structure of Human APOBEC3A complexed with ssDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-30
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 2 2 21
Unit cell lengths	90.148, 90.199, 167.257
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.424 - 3.151
R-factor	0.2141
Rwork	0.210
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4XXO Chain A
RMSD bond length	0.002
RMSD bond angle	0.455
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((dev_2499: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.150	3.150
Rmerge		1.120
Number of reflections	24189
<I/σ(I)>	8.4	1.44
Completeness [%]	100.0	99
Redundancy	5.5	5.6
CC(1/2)		0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	NaF, PEG3350