5S5U

Tubulin-Z1124201124-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.780, 158.650, 178.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.330 - 2.500
R-factor	0.2121
Rwork	0.210
R-free	0.25580
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	79.330	79.330	2.560
High resolution limit [Å]	2.500	11.180	2.500
Rmerge	0.110	0.030	2.746
Rmeas	0.119	0.033	2.971
Total number of observations	700397
Number of reflections	103364	1289	7557
<I/σ(I)>	10.67	52.72	0.64
Completeness [%]	99.8	98.8	99.6
Redundancy	6.776	6.335	6.828
CC(1/2)	0.998	0.998	0.271

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine