5S5Q

Tubulin-Z396380540-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.850, 158.620, 178.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	90.420 - 2.050
R-factor	0.2136
Rwork	0.212
R-free	0.24400
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	90.420	90.420	2.100
High resolution limit [Å]	2.050	9.170	2.050
Rmerge	0.081	0.031	2.134
Rmeas	0.088	0.033	2.352
Total number of observations	1239010
Number of reflections	186150	2294	13655
<I/σ(I)>	11.63	48.17	0.76
Completeness [%]	99.8	99.4	99.7
Redundancy	6.656	6.081	5.728
CC(1/2)	0.999	0.999	0.334

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine