5S5N

Tubulin-Z165170770-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	105.610, 159.430, 179.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.620 - 2.900
R-factor	0.2245
Rwork	0.222
R-free	0.27350
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.620	63.620	2.980
High resolution limit [Å]	2.900	12.970	2.900
Rmerge	0.149	0.021	2.517
Rmeas	0.162	0.022	2.718
Total number of observations	441656
Number of reflections	66075	861	4951
<I/σ(I)>	9.98	59.92	0.84
Completeness [%]	97.4	99	99.9
Redundancy	6.684	6.092	7.036
CC(1/2)	0.999	1.000	0.491

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine