5S5K

Tubulin-Z2472938267-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	105.460, 160.780, 178.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.280 - 2.410
R-factor	0.216
Rwork	0.214
R-free	0.24780
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	73.280	73.280	2.470
High resolution limit [Å]	2.410	10.780	2.410
Rmerge	0.098	0.033	2.641
Rmeas	0.106	0.036	2.854
Total number of observations	779690
Number of reflections	115926	1456	8562
<I/σ(I)>	10.62	46.72	0.75
Completeness [%]	98.8	98.8	99.7
Redundancy	6.726	6.34	6.959
CC(1/2)	0.999	0.999	0.326

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine