5S5F

Tubulin-Z87615031-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.828, 158.374, 179.214
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.430 - 2.240
R-factor	0.2359
Rwork	0.236
R-free	0.26170
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	72.430	72.430	2.300
High resolution limit [Å]	2.240	10.020	2.240
Rmerge	0.136	0.079	1.932
Rmeas	0.148	0.086	2.110
Total number of observations	981011
Number of reflections	144973	1787	10540
<I/σ(I)>	7.58	21.42	0.87
Completeness [%]	99.9	99.3	99.5
Redundancy	6.767	6.193	6.235
CC(1/2)	0.994	0.993	0.591

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine