5S5C

Tubulin-Z44592329-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	105.220, 159.900, 177.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.930 - 2.400
R-factor	0.2091
Rwork	0.207
R-free	0.24390
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	67.930	67.930	2.460
High resolution limit [Å]	2.400	10.730	2.400
Rmerge	0.116	0.027	2.277
Rmeas	0.126	0.029	2.460
Total number of observations	797912
Number of reflections	117490	1460	8604
<I/σ(I)>	10.22	51.45	0.82
Completeness [%]	99.8	99.1	99.8
Redundancy	6.791	6.373	6.99
CC(1/2)	0.999	0.999	0.383

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine