5S53

Tubulin-Z1349163663-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	105.320, 159.310, 178.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.190 - 2.750
R-factor	0.22
Rwork	0.218
R-free	0.26690
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	68.190	68.190	2.820
High resolution limit [Å]	2.750	12.300	2.750
Rmerge	0.126	0.019	2.542
Rmeas	0.137	0.021	2.750
Total number of observations	524219
Number of reflections	77724	990	5699
<I/σ(I)>	11.24	65.17	0.79
Completeness [%]	98.5	98.6	98.7
Redundancy	6.745	6.273	6.89
CC(1/2)	0.999	1.000	0.466

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine