5S4O

Tubulin-Z48847594-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.744, 158.001, 178.586
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.000 - 2.300
R-factor	0.2164
Rwork	0.216
R-free	0.26170
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	79.000	79.000	2.360
High resolution limit [Å]	2.300	10.290	2.300
Rmerge	0.133	0.072	1.731
Rmeas	0.144	0.079	1.878
Total number of observations	905186
Number of reflections	133616	1661	9784
<I/σ(I)>	7.51	20.76	1.01
Completeness [%]	100.0	99.3	100
Redundancy	6.775	6.128	6.647
CC(1/2)	0.995	0.995	0.473

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine