5Q0M
Ligand binding to FARNESOID-X-RECEPTOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 |
| Unit cell lengths | 93.562, 93.562, 45.590 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.514 - 2.200 |
| R-factor | 0.1869 |
| Rwork | 0.183 |
| R-free | 0.26150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.024 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.800 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.062 | 0.543 |
| Number of reflections | 11752 | 1454 |
| <I/σ(I)> | 12.61 | 1.86 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 4.14 | 3.59 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | evaporation, hanging drop | 7.5 | 298 | 0.1 M HEPES pH 7.5, 1.4 M Sodium Citrate |
