5OWR
Human STK10 bound to dasatinib
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-05 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 49.740, 111.720, 133.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 85.580 - 2.300 |
| R-factor | 0.22146 |
| Rwork | 0.220 |
| R-free | 0.25362 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | STK10 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.180 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.580 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 1.030 |
| Number of reflections | 16890 | 7139 |
| <I/σ(I)> | 37.2 | 1.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.3 | 4.3 |
| CC(1/2) | 0.997 | 0.667 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 40% PEG 300, 0.20M Ca(ac)2, 0.1M cacodylate pH 6.5 |
