5OWE

Crystal structure of the human BRPF1 bromodomain in complex with DSPBP1010

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000020
Spacegroup name	P 32 2 1
Unit cell lengths	60.530, 60.530, 63.680
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.300 - 1.700
R-factor	0.1927
Rwork	0.190
R-free	0.21340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lc2
RMSD bond length	0.007
RMSD bond angle	0.744
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.300	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.050	0.540
Number of reflections	15062	794
<I/σ(I)>	31.1
Completeness [%]	98.9	98.2
Redundancy	19.8	20.9
CC(1/2)	0.998	0.956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M Bis-tris propane, pH6.5, 0.2 M Sodium nitrate, 20% PEG3350