5OWB
Crystal structure of the human BRPF1 bromodomain in complex with DSPBP1004
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-27 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.000020 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 60.426, 60.426, 63.468 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.376 - 1.650 |
R-factor | 0.1776 |
Rwork | 0.175 |
R-free | 0.20440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lc2 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.831 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.380 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.067 | 0.627 |
Number of reflections | 16528 | 815 |
<I/σ(I)> | 26.7 | 4.3 |
Completeness [%] | 99.8 | |
Redundancy | 19.3 | |
CC(1/2) | 0.999 | 0.924 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1M Sodium Acetate, pH5.5, 0.15 M Sodium Nitrate, 20% PEG3350 |