5OVT
Thiobacillus denitrificans BPH in complex with Epoxomicin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-21 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 196.820, 196.820, 296.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.200 - 2.950 |
| R-factor | 0.17323 |
| Rwork | 0.172 |
| R-free | 0.18860 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.111 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.200 | 3.120 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.439 | 1.980 |
| Number of reflections | 71632 | 10916 |
| <I/σ(I)> | 13.6 | 3.11 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 57.1 | 57.6 |
| CC(1/2) | 0.995 | 0.878 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 294 | 100 mM sodium acetate pH 4.8, 1.2 M sodium nitrate |
