5OTW
Extracellular domain of GLP-1 receptor in complex with GLP-1 variant Ala8Hcs/Thr11Cys
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-X |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-01 |
Detector | DECTRIS PILATUS3 R 1M |
Wavelength(s) | 1.54187 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.260, 81.200, 42.740 |
Unit cell angles | 90.00, 98.67, 90.00 |
Refinement procedure
Resolution | 42.252 - 2.100 |
R-factor | 0.189 |
Rwork | 0.184 |
R-free | 0.23920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zgm |
RMSD bond length | 0.005 |
RMSD bond angle | 0.723 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2689: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.260 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.107 | 0.980 |
Rmeas | 1.086 | |
Rpim | 0.049 | 0.459 |
Number of reflections | 15304 | |
<I/σ(I)> | 11.9 | 1.9 |
Completeness [%] | 98.5 | 95.64 |
Redundancy | 5.6 | 5.53 |
CC(1/2) | 0.997 | 0.742 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Morpheus from Molecular Dimensions, solution E12: 0.12 M ethylene Glycols, 0.1 M Buffer system 3 pH 8.5, 12.5% (v/v) MPD, 12.5% (w/v) PEG1000, 12.5% (w/v) PEG3350 |