5OTU
Extracellular domain of GLP-1 receptor in complex with GLP-1 variant Ala8Hcs/Thr11Hcs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-04 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.822, 81.038, 42.945 |
| Unit cell angles | 90.00, 97.92, 90.00 |
Refinement procedure
| Resolution | 42.535 - 1.800 |
| R-factor | 0.1747 |
| Rwork | 0.173 |
| R-free | 0.21060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zgm |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.840 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2689: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.535 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.045 | 0.482 |
| Rmeas | 0.568 | |
| Rpim | 0.029 | 0.298 |
| Number of reflections | 24985 | |
| <I/σ(I)> | 15.6 | 2.7 |
| Completeness [%] | 99.7 | 99.68 |
| Redundancy | 3.4 | 3.53 |
| CC(1/2) | 0.999 | 0.851 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Morpheus from Molecular Dimensions, solution G1: 0.1 M Carboxylic acids, 0.1 Buffer system 1 pH 6.5, 20% (v/v) PEG500mme, 10% (w/v) PEG20000 |
