5OSK

Tubulin-7j complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-09
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	105.670, 159.920, 181.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.792 - 2.110
R-factor	0.1969
Rwork	0.195
R-free	0.23200
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
RMSD bond length	0.007
RMSD bond angle	0.858
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.800	2.160
High resolution limit [Å]	2.100	2.100
Number of reflections	172614	12620
<I/σ(I)>	19.5	1.02
Completeness [%]	99.9	99.8
Redundancy	6.7	6.6
CC(1/2)	1.000	0.394

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.7	293	10% PEG 4K, 16% glycerol, 30 mM MgCl2, 30 mM CaCl2, and 100 mM MES/Imidazole (pH 6.7)