5OSI
Structure of retromer VPS29-VPS35C subunits complexed with RidL harpin loop (163-176)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979070 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.554, 138.993, 126.637 |
| Unit cell angles | 90.00, 92.78, 90.00 |
Refinement procedure
| Resolution | 126.490 - 2.520 |
| R-factor | 0.2079 |
| Rwork | 0.205 |
| R-free | 0.26680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r17 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.244 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 126.490 | 126.650 | 2.560 |
| High resolution limit [Å] | 2.520 | 6.840 | 2.520 |
| Rmeas | 0.155 | 0.057 | 1.134 |
| Rpim | 0.062 | 0.023 | 0.455 |
| Total number of observations | 425544 | ||
| Number of reflections | 68126 | 3506 | 3399 |
| <I/σ(I)> | 8.5 | 20.3 | 1.9 |
| Completeness [%] | 99.8 | 100 | 99.7 |
| Redundancy | 6.2 | 6.1 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1 M NaCl, 20% PEG3350 0.1 M Tris pH 8.5 |
