5OSA
GLIC-GABAAR alpha1 chimera crystallized at pH4.6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 185.017, 133.947, 162.697 |
| Unit cell angles | 90.00, 103.53, 90.00 |
Refinement procedure
| Resolution | 29.980 - 2.750 |
| R-factor | 0.20463086704 |
| Rwork | 0.203 |
| R-free | 0.22886 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hfi |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.813 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (dev_2645) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.280 | 2.800 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.068 | 0.772 |
| Rpim | 0.066 | 0.730 |
| Number of reflections | 100157 | 4988 |
| <I/σ(I)> | 1.2 | 1.2 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.946 | 0.868 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 289.15 | 100 mM NaCl, 100 mM sodium acetate pH 4.6, 12 % PEG 6000 |
