5OQI
Crystal Structure of a disulfide trapped single chain trimer composed of the MHC I heavy chain H-2Kb Y84C E63A mutant, beta-2microglobulin, and ovalbumin-derived peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-02 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.130, 90.190, 89.450 |
| Unit cell angles | 90.00, 111.22, 90.00 |
Refinement procedure
| Resolution | 42.517 - 2.400 |
| R-factor | 0.2034 |
| Rwork | 0.201 |
| R-free | 0.25950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qri |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.541 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.520 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 38095 |
| <I/σ(I)> | 11.6 |
| Completeness [%] | 97.9 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.2M Am sulphate, 25% PEG 4K, 0.1M Na cacodylate pH 6.5 |
