5OQG
Crystal structure of a single chain trimer composed of the MHC I heavy chain H-2Kb W167A, beta-2microglobulin, and and ovalbumin-derived peptide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-28 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.430, 89.640, 89.450 |
| Unit cell angles | 90.00, 111.24, 90.00 |
Refinement procedure
| Resolution | 61.506 - 1.900 |
| R-factor | 0.1905 |
| Rwork | 0.188 |
| R-free | 0.23880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qri |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.878 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 61.510 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 76853 |
| <I/σ(I)> | 14.3 |
| Completeness [%] | 99.8 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M Am sulphate, 23& PEG 8K, 0.1M Bis-tris, pH 6.5 |
