5OPM
Crystal structure of D52N/R238W cN-II mutant bound to dATP and free phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 92.087, 127.454, 130.948 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.670 - 1.680 |
| R-factor | 0.1784 |
| Rwork | 0.178 |
| R-free | 0.19660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5k7y |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.481 |
| Data reduction software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.670 | 1.780 |
| High resolution limit [Å] | 1.677 | 1.680 |
| Rmerge | 0.058 | 0.645 |
| Rmeas | 0.068 | 0.751 |
| Number of reflections | 84931 | 13693 |
| <I/σ(I)> | 14.5 | 1.95 |
| Completeness [%] | 99.1 | 97.3 |
| Redundancy | 3.7 | 3.8 |
| CC(1/2) | 0.999 | 0.663 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 M bicine/Trizma base pH 8.5; 0.02 M of each amino acid; 10% w/v PEG 4000, 20% v/v glycerol H11 Morpheus condition |
