5OKP
Crystal structure of human SHIP2 Phosphatase-C2 double mutant F593D/L597D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 43.745, 73.426, 157.964 |
| Unit cell angles | 90.00, 90.70, 90.00 |
Refinement procedure
| Resolution | 78.980 - 1.850 |
| R-factor | 0.17923 |
| Rwork | 0.178 |
| R-free | 0.20469 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.260 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.980 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.059 | 0.595 |
| Rpim | 0.038 | 0.372 |
| Number of reflections | 40283 | |
| <I/σ(I)> | 11.6 | 2.1 |
| Completeness [%] | 99.3 | 99.1 |
| Redundancy | 3.4 | 3.5 |
| CC(1/2) | 0.999 | 0.768 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 293 | 50 mM HEPES pH 7.1, 17.5% PEG 1000 |
