5OKM

Crystal structure of human SHIP2 Phosphatase-C2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-06-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	136.037, 175.836, 176.893
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.960 - 1.960
R-factor	0.18091
Rwork	0.179
R-free	0.20750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nr8
RMSD bond length	0.009
RMSD bond angle	1.306
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.970	1.990
High resolution limit [Å]	1.960	1.960
Rpim	0.033	0.368
Number of reflections	287002
<I/σ(I)>	14.8	2.1
Completeness [%]	100.0	100
Redundancy	6.8	6.7
CC(1/2)	0.999	0.743

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	0.1 M BisTris propane pH 7, 0.2 M NaNO3, 20% PEG 3350, 0.025% CH2Cl2, 2 mM TCEP