5OJJ
Crystal structure of the Zn-bound ubiquitin-conjugating enzyme Ube2T
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-25 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9282 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.020, 96.383, 89.996 |
| Unit cell angles | 90.00, 93.28, 90.00 |
Refinement procedure
| Resolution | 48.750 - 1.850 |
| R-factor | 0.17915 |
| Rwork | 0.178 |
| R-free | 0.19885 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yh2 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.474 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.750 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.038 | 0.292 |
| Number of reflections | 81137 | 4465 |
| <I/σ(I)> | 16.6 | 3.1 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 3.4 | 3.1 |
| CC(1/2) | 0.998 | 0.849 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10% PEG3350, 0.2 M calcium acetate, 0.1 M Tris pH 8.5 |
