5OGK
Crystal structure of a nucleotide sugar transporter with bound nucleotide sugar.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 83 |
| Detector technology | PIXEL |
| Collection date | 2017-05-26 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.319, 101.694, 180.192 |
| Unit cell angles | 89.86, 90.07, 90.11 |
Refinement procedure
| Resolution | 44.328 - 3.600 |
| R-factor | 0.2597 |
| Rwork | 0.257 |
| R-free | 0.31270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5oge |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.652 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.300 | 3.760 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rpim | 0.200 | 1.110 |
| Number of reflections | 36110 | 4518 |
| <I/σ(I)> | 3.8 | 0.6 |
| Completeness [%] | 96.8 | 97.2 |
| Redundancy | 1.8 | 1.8 |
| CC(1/2) | 0.980 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5 | 277 | 26 - 30 % (v/v) PEG 400, 0.1 M sodium citrate pH 5.0 and 75 mM sodium chloride or sodium acetate. GDP-mannose was soaked in at 20mM overnight. |
