5OC9

Crystal Structure of human TMEM16K / Anoctamin 10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 21 21 21
Unit cell lengths	59.150, 153.590, 218.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.790 - 3.200
R-factor	0.2292
Rwork	0.228
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wis
RMSD bond length	0.008
RMSD bond angle	0.870
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MrBUMP
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.790	3.280
High resolution limit [Å]	3.200	3.200
Rmerge	0.285	1.265
Rpim	0.139	0.610
Number of reflections	33892	2488
<I/σ(I)>	5.9	1.4
Completeness [%]	99.9	99.9
Redundancy	5.1	5.2
CC(1/2)	0.990	0.535

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6	293	0.1M MES pH 6.0 -- 0.1M sodium chloride -- 0.1M calcium chloride -- 30% PEG300