5OC8
HDM2 (17-111, WILD TYPE) COMPLEXED WITH NVP-HDM201 AT 1.56A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-01-26 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.99986 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 56.406, 56.406, 104.913 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.210 - 1.560 |
| R-factor | 0.2382 |
| Rwork | 0.237 |
| R-free | 0.26030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ln2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.220 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.210 | 19.210 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.055 | 0.833 |
| Rmeas | 0.057 | 0.858 |
| Rpim | 0.054 | 0.422 |
| Number of reflections | 14706 | |
| <I/σ(I)> | 31.6 | 4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 18.4 | 18.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 3M AmSO4, 0.1M citrate |
