5OC5

Crystal structure of human tRNA-dihydrouridine(20) synthase dsRBD K419A-K420A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-05
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98011
Spacegroup name	P 41
Unit cell lengths	36.170, 36.170, 75.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.170 - 1.893
R-factor	0.1747
Rwork	0.169
R-free	0.22620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wft
RMSD bond length	0.015
RMSD bond angle	1.138
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.170	1.961
High resolution limit [Å]	1.893	1.893
Rmerge	0.099
Number of reflections	7680	710
<I/σ(I)>	13.3	1.29
Completeness [%]	99.5	96.33
Redundancy	9.4	7.7
CC(1/2)	0.998	0.468

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	292	35% PEG 4K 1 M LiCl 100 mM Tris