5OBM
Crystal structure of Gentamicin bound to the yeast 80S ribosome
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2015-07-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 436.630, 287.000, 304.730 |
| Unit cell angles | 90.00, 99.08, 90.00 |
Refinement procedure
| Resolution | 143.721 - 3.400 |
| R-factor | 0.1896 |
| Rwork | 0.189 |
| R-free | 0.23790 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.226 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 143.721 | 143.721 | 3.500 |
| High resolution limit [Å] | 3.400 | 105.000 | 3.400 |
| Rmerge | 0.297 | 1.554 | |
| Rmeas | 0.308 | 1.751 | |
| Number of reflections | 1013190 | 83925 | |
| <I/σ(I)> | 9.21 | 0.98 | |
| Completeness [%] | 99.9 | 99.6 | |
| Redundancy | 15.171 | 4.683 | |
| CC(1/2) | 0.995 | 0.355 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | PEG 20K, KSCN, Mg Acetate, Tris-Acetate, Glycerol, Spermidine |
