5OAE

Crystal Structure of tyrosinase from Bacillus megaterium with SVF inhibitor in the active site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	78.450, 84.100, 89.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.956 - 2.700
R-factor	0.2185
Rwork	0.216
R-free	0.26090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4p6r
RMSD bond length	0.003
RMSD bond angle	0.731
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.590	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.107	0.713
Rpim	0.060	0.378
Number of reflections	15340	2286
<I/σ(I)>	10.9	2.6
Completeness [%]	92.0	95.6
Redundancy	3.9	4.1
CC(1/2)	0.996	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	PEG 8000, sodium cacodylate