5OAB

A novel crystal form of human RNase6 at atomic resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	I 2 3
Unit cell lengths	95.405, 95.405, 95.405
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.703 - 1.111
R-factor	0.1184
Rwork	0.117
R-free	0.14120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4x09
RMSD bond length	0.009
RMSD bond angle	1.102
Data reduction software	XDS (20151001)
Data scaling software	Aimless (0.5.17)
Phasing software	PHENIX (1.11.1_2575)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.703	1.151
High resolution limit [Å]	1.111	1.111
Rmerge	0.082	0.656
Rmeas	0.094	0.813
Rpim	0.044	0.469
Number of reflections	54593	4253
<I/σ(I)>	9.1	1.47
Completeness [%]	96.2	75.17
Redundancy	4	2.4
CC(1/2)	0.996	0.544

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.3 M SODIUM/POTASSIUM PHOSPHATE