5O9J
Crystal structure of transcription factor IIB Mja mini-intein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.360, 50.798, 60.118 |
| Unit cell angles | 77.42, 75.83, 76.93 |
Refinement procedure
| Resolution | 28.700 - 2.000 |
| R-factor | 0.2153 |
| Rwork | 0.214 |
| R-free | 0.26120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cw8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.013 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MR-Rosetta |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.700 | |
| High resolution limit [Å] | 2.000 | 2.070 |
| Rmerge | 0.085 | 0.724 |
| Number of reflections | 30100 | |
| <I/σ(I)> | 11.6 | 1.6 |
| Completeness [%] | 95.9 | 92.5 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | MES, dioxane, ammonium sulfate |
