5O8Y
Conformational dynamism for DNA interaction in Salmonella typhimurium RcsB response regulator.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-14 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 108.830, 108.830, 306.538 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 102.560 - 2.300 |
| R-factor | 0.22484 |
| Rwork | 0.223 |
| R-free | 0.25274 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a04 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.186 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 102.600 | 2.264 |
| High resolution limit [Å] | 2.256 | 2.256 |
| Rmerge | 0.175 | |
| Rmeas | 0.179 | 2.958 |
| Rpim | 0.035 | 0.652 |
| Number of reflections | 87626 | 845 |
| <I/σ(I)> | 17.9 | |
| Completeness [%] | 100.0 | 92.2 |
| Redundancy | 20 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 1.4M lithium sulfate 8% PEG1000 Hepes pH 7.5 |
