5O8F
Structure of a chimaeric beta3-alpha5 GABAA receptor in complex with nanobody Nb25 and pregnanolone
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-26 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | I 2 3 |
Unit cell lengths | 290.102, 290.102, 290.102 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 205.130 - 3.200 |
R-factor | 0.23296 |
Rwork | 0.232 |
R-free | 0.24240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4cof |
RMSD bond length | 0.007 |
RMSD bond angle | 1.121 |
Data reduction software | HKL-2000 |
Data scaling software | Aimless (0.5.25) |
Phasing software | PHASER (2.5.7) |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.752 | 3.310 |
High resolution limit [Å] | 3.190 | 3.190 |
Rmerge | 0.621 | 3.131 |
Rpim | 0.131 | 0.609 |
Number of reflections | 66928 | 6677 |
<I/σ(I)> | 6.8 | 1.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 30.4 | 25.5 |
CC(1/2) | 0.986 | 0.215 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | 14% PEG 6000, 0.1M N-(2-Acetamido)iminodiacetic acid (ADA), 7.6 mM 4-Cyclohexyl-1-Butyl-beta-D-Maltoside (CYMAL-4) |