5O7P

HER3 in complex with Fab MF3178

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97625
Spacegroup name	P 64 2 2
Unit cell lengths	141.131, 141.131, 320.817
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.987 - 4.500
R-factor	0.2497
Rwork	0.247
R-free	0.29600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m6b 3skj 4hcr
RMSD bond length	0.010
RMSD bond angle	0.729
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11_2567: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.990	5.000
High resolution limit [Å]	4.500	4.500
Rmerge	0.200	1.400
Rpim	0.066	0.460
Number of reflections	11931	3286
<I/σ(I)>	9.6	2.2
Completeness [%]	99.9	100
Redundancy	10.9	11.3
CC(1/2)	0.998	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M bis tris propane pH 7.5, 0.15 sodium citrate, 20% w/v PEG 3350