5O75
Ube4B U-box domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2016-12-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97628 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 80.180, 80.180, 39.525 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.090 - 1.483 |
| R-factor | 0.1966 |
| Rwork | 0.195 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3l1z |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.029 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.438 | 1.509 |
| High resolution limit [Å] | 1.483 | 1.483 |
| Rmerge | 0.043 | 1.194 |
| Rpim | 0.015 | 0.435 |
| Number of reflections | 11868 | 636 |
| <I/σ(I)> | 27.9 | 2.2 |
| Completeness [%] | 91.9 | 100 |
| Redundancy | 17 | 15 |
| CC(1/2) | 1.000 | 0.771 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M citric acid, pH 5.5, 1.9 M ammonium sulphate |
