5O74
Crystal structure of human Rab1b covalently bound to the GEF domain of DrrA/SidM from Legionella pneumophila in the presence of GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00005 |
| Spacegroup name | P 1 |
| Unit cell lengths | 77.910, 87.450, 93.170 |
| Unit cell angles | 114.61, 97.69, 100.74 |
Refinement procedure
| Resolution | 46.525 - 2.500 |
| R-factor | 0.2304 |
| Rwork | 0.229 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jza |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.952 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.525 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 72360 | 7978 |
| <I/σ(I)> | 10.2 | 2.5 |
| Completeness [%] | 97.8 | 97.5 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 19% PEG 600, 100mM NaCitrate pH 5.5, protein concentration 22mg/ml |
