5O5F
Crystal structure of the human BRPF1 bromodomain in complex with BZ038
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.765, 60.765, 63.516 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.382 - 1.302 |
| R-factor | 0.176 |
| Rwork | 0.175 |
| R-free | 0.18780 |
| Starting model (for MR) | 4lc2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.779 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.520 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.022 | 0.437 |
| Number of reflections | 33637 | 1591 |
| <I/σ(I)> | 37.3 | 3.1 |
| Completeness [%] | 99.8 | 96.4 |
| Redundancy | 7.3 | 4.6 |
| CC(1/2) | 1.000 | 0.802 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M Bis-tris propane, pH6.5, 0.2 M Sodium Nitrate, 20% PEG3350 |
