5O4Y
Structure of human PD-L1 in complex with inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.550, 80.950, 54.230 |
Unit cell angles | 90.00, 104.49, 90.00 |
Refinement procedure
Resolution | 29.780 - 2.300 |
R-factor | 0.201 |
Rwork | 0.198 |
R-free | 0.26200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5c3t |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.780 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.107 | 0.552 |
Number of reflections | 18522 | |
<I/σ(I)> | 6.6 | |
Completeness [%] | 97.9 | |
Redundancy | 3 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M ammonium acetate (pH 5.5), 0.1 M Bis-Tris, 25% PEG 3350 |