5O4O
HER3 in complex with Fab MF3178
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-24 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.96771 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 64.380, 144.020, 218.756 |
Unit cell angles | 90.00, 97.91, 90.00 |
Refinement procedure
Resolution | 59.971 - 3.400 |
R-factor | 0.2171 |
Rwork | 0.215 |
R-free | 0.26100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m6b 3skj 4hcr |
RMSD bond length | 0.002 |
RMSD bond angle | 0.514 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.970 | 3.600 |
High resolution limit [Å] | 3.400 | 3.400 |
Rpim | 0.134 | 0.372 |
Number of reflections | 27028 | 4355 |
<I/σ(I)> | 17.7 | 2.5 |
Completeness [%] | 99.2 | 99.4 |
Redundancy | 3.3 | 3.2 |
CC(1/2) | 0.942 | 0.691 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M bis tris propane pH 8.5, 0.2 M potassium thyocianate and 20% w/v PEG 3350 |