5O49
Human FGF in complex with a covalent inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 209.550, 57.590, 65.620 |
| Unit cell angles | 90.00, 107.64, 90.00 |
Refinement procedure
| Resolution | 49.920 - 1.910 |
| R-factor | 0.2 |
| R-free | 0.22000 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.920 | 1.960 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.036 | 0.735 |
| Number of reflections | 57050 | 4147 |
| <I/σ(I)> | 13.9 | 1.5 |
| Completeness [%] | 98.2 | 97.8 |
| Redundancy | 3.2 | 2.8 |
| CC(1/2) | 0.998 | 0.548 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 18-20% (w/v) PEG8000, 200mM ammonium sulphate, 100mM PCTP pH 6.75 and 20% (v/v) ethylene glycol |
