5O3G

Human Brd2(BD2) mutant in complex with AL-Am1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9282
Spacegroup name	P 21 21 2
Unit cell lengths	73.740, 52.660, 63.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.412 - 1.850
R-factor	0.1899
Rwork	0.188
R-free	0.23630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qeu
RMSD bond length	0.014
RMSD bond angle	1.516
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.412	1.916
High resolution limit [Å]	1.850	1.850
Rmerge	0.076	0.388
Number of reflections	20578	2034
<I/σ(I)>	9.64
Completeness [%]	95.2	95.67
Redundancy	3.9	3.9
CC(1/2)	0.997	0.776

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.25	293	0.1M Tris pH 8.25 55% Pentaerythritol propoxylate (5/4 PO/OH)