5O3A
Human Brd2(BD2) mutant in complex with ET
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-19 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 126.799, 126.799, 43.194 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.097 - 2.400 |
R-factor | 0.208 |
Rwork | 0.205 |
R-free | 0.25470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qeu |
RMSD bond length | 0.011 |
RMSD bond angle | 1.192 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.100 | 2.486 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.100 | 0.331 |
Number of reflections | 7114 | 696 |
<I/σ(I)> | 17.11 | 5.14 |
Completeness [%] | 99.2 | 100 |
Redundancy | 15.3 | 14.6 |
CC(1/2) | 0.999 | 0.996 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.25 | 293 | 0.1M Tris pH 8.25 55% Pentaerythritol propoxylate (5/4 PO/OH) 0.2M Imidazole |